Function Analysis of Ribophorin Ⅱ Gene in the Brown Planthopper (Nilaparvata lugens)
WANG Sai-Nan1, ZHANG Chuan-Xi1,2, LU Jia-Bao1,*
1 Institute of Plant Virology State/Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products/Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs and Zhejiang Province, Ningbo University, Ningbo 315211, China; 2 Institute of Insect Science, Zhejiang University, Hangzhou 310058, China
Abstract：Ribophorin Ⅱ (RⅡ) is a crucial subunit of the oligosaccharide transferase, and studies have shown that RⅡ is associated with cancer occurrence in humans (Homo sapiens), but there is currently limited research on the function of RⅡ in insects. In this study, a gene encoding RⅡ was identified in the brown planthopper (Nilaparvata lugens, BPH), a rice (Oryza sativa) pest, and is named N. lugens ribophorin Ⅱ (NlRⅡ)(GenBank No. XP_022202517.1). To investigate the function of NlRⅡ in BPH, the temporal expression pattern of NlRⅡ using transcriptomic data was first obtained, and the result revealed the expression pattern of NlRⅡ changed periodically with molting and reached a peak in each mid-instar stage. Then, qRT-PCR was used to detect the tissue-specific expression of NlRⅡ, the result showed that the expression level of NlRⅡ was highest in the ovary. Finally, RNA interference (RNAi) technique was used to silence NlRⅡ in the 2nd instar nymphs, and no significant effect was found on the survival of BPH. However, silencing NlRⅡ in the 5th instar resulted in female adult abdominal swelling, malformed ovary development, and almost no egg production. Additionally, significant decreases in egg number and hatchability were observed in wild-type female adults mated with male adults with extremely significantly constricted vas deferens (P<0.01). This study indicated that NlRⅡ could affect the reproduction of BPH, and provide a potential new target gene for RNAi-based BPH control.
王赛男, 张传溪, 鲁嘉宝. 褐飞虱核糖体结合蛋白Ⅱ基因的功能分析[J]. 农业生物技术学报, 2023, 31(12): 2556-2567.
WANG Sai-Nan, ZHANG Chuan-Xi, LU Jia-Bao. Function Analysis of Ribophorin Ⅱ Gene in the Brown Planthopper (Nilaparvata lugens). 农业生物技术学报, 2023, 31(12): 2556-2567.
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