Abstract：As highly competitive endogenous RNA (competing endogenous RNA, ceRNA), circular RNAs (circRNAs) regulate cell differentiation, proliferation, apoptosis and energy metabolism through the sponge effect of miRNA. Lycium ruthenicum is rich in anthocyanin, which has remarkable antioxidant activity and can effectively reduce the hypoxic injury of cardiomyocytes. In this study, RNA-seq technology and bioinformatics methods were used to screen differentially expressed circRNA, miRNA and mRNA, and construct circRNA- miRNA-mRNA regulatory network. qRT-PCR was used to screen ceRNA relationship pairs that might be involved in the regulation of L. ruthenicum anthocyanins in hypoxic-induced H9c2 rat cardiomyocytes. The results showed that the differentially expressed 1 570 mRNAs, 5 miRNAs and 4 circRNAs were hypoxic- related molecules, which might be involved in the regulation of L. ruthenicum anthocyanins in hypoxic- induced H9c2 rat (Rattus norvegicus) cardiomyocytes. Based on pearson correlation analysis and target relationship prediction, the ceRNA network was successfully constructed, and 2 candidate circRNA-miRNA- mRNA relationship pairs were screened by qRT-PCR. This study provides basic data to further explore the mechanism of circRNA and its target genes involved in regulating the protective effect of anthocyanin in L. barbarum on cardiomyocytes.
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