Effects of Porcine epidemic diarrhea virus Infection on Intestinal Mucosal Barrier and the Expression of Adherens Junction Protein Genes in Piglets(Sus scrofa)
DU Yu-Lu, ZHOU Ya-Jing, WANG Hai-Fei, WU Zheng-Chang, WU Sheng-Long, BAO Wen-Bin*
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Abstract：Porcine epidemic diarrhea virus (PEDV) is a common pathogen that causes diarrhea in piglets (Sus scrofa). The intestinal mucosal barrier is destroyed after PEDV-infected, which leads to diarrhea in piglets. Adherens junction is an important part of the intestinal mucosal barrier. In order to investigate the effects of PEDV on the intestinal mucosal barrier and expression of adherens junction protein genes in piglets, the effect of PEDV on the intestinal mucosa of piglets were observed by electron microscopy in paraffin sections in this study. The expression of E-cadherin, Integrin and Nectin-1 genes in the intestines of PEDV-infected and normal piglets was examined by qRT-PCR, as well as in PEDV infected intestinal porcine epithelial cell line J2 (IPEC-J2). And their relationship with intestinal barrier function was further analyzed. The results showed that the intestinal villi of PEDV-infected piglets were damaged and shed, the lamina propria was exposed and the intestinal glands were atrophied, while the intestinal mucosal barrier structure of normal piglets were intact and distinct. The results of qRT-PCR indicated that the expression of E-cadherin gene in duodenum of PEDV-infected piglets was significantly lower than that of normal piglets (P<0.05), and the expression of Nectin-1 gene in jejunum was significantly lower than that of normal piglets (P<0.01). There was no significant difference in other groups. In vitro cell infection experiments also showed that the expression of E-cadherin and Nectin-1 genes was significantly reduced after IPED-J2 cells infected by PEDV. These results revealed that the intestinal mucosal barrier of epidemic diarrhea piglets was damaged and the adherens junction was destroyed. The down-regulation of E-cadherin and Nectin-1 was not conducive to maintain the integrity of intestinal mucosal barrier, and the damage of intestinal mucosal barrier might further promote the occurrence of diarrhea in piglets. This study initially revealed the effects of PEDV infection on intestinal mucosal barrier and adherens junction of piglets, and provides a new insights into the pathogenic mechanism of PEDV from the perspective of the intestinal barrier.
杜雨露, 周雅静, 王海飞, 吴正常, 吴圣龙, 包文斌. 猪流行性腹泻病毒对仔猪小肠黏膜屏障以及粘附连接蛋白基因表达的影响[J]. 农业生物技术学报, 2021, 29(1): 85-92.
DU Yu-Lu, ZHOU Ya-Jing, WANG Hai-Fei, WU Zheng-Chang, WU Sheng-Long, BAO Wen-Bin. Effects of Porcine epidemic diarrhea virus Infection on Intestinal Mucosal Barrier and the Expression of Adherens Junction Protein Genes in Piglets(Sus scrofa). 农业生物技术学报, 2021, 29(1): 85-92.
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