Genome-wide Linkage Analysis and Breeding Marker Development for Coleoptile Length in Common Wheat (Triticum aestivum)
WANG Li-Na1, ZHANG Qian1, LIU Jin-Dong2, LI Nan-Nan1, LYU Yong-Jun1, ZOU Shao-Kui1, LI Shun-Cheng1, DU Xiao-Yu1, YU Hai-Fei1, LI Yong-Gang1, HUANG Zhi-Bo1, HAN Yu-Lin1,*
1 Zhoukou Academy of Agricultural Sciences, Zhoukou 466000, China; 2 Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Abstract：Drought stress is a major limiting factor for stable and high wheat (Triticum aestivum) production. Under drought conditions, appropriate deep sowing enables seeds to access deeper soil moisture, thereby improving seedling emergence rate. Coleoptile length determines the maximum sowing depth of wheat; therefore, mining elite genetic resources controlling coleoptile length and developing available molecular markers are of great significance for genetic improvement of deep-sowing tolerance and drought resistance. In this study, a recombinant inbred line (RIL) population derived from 'Gaocheng 8901' and 'Zhoumai 16' was used to evaluate coleoptile length. Genotyping was performed using the wheat 90K SNP array. A total of 4 QTL associated with coleoptile length were detected on chromosomes 1AL, 2BS, 3BS and 7DL through genome-wide linkage analysis, designated as QCL.zaas-1AL, QCL.zaas-2BS, QCL.zaas-3BS and QCL.zaas-7DL, with phenotypic variation explained (PVE) ranging from 7.12% to 7.89%. The favorable alleles of QCL.zaas-1AL, QCL.zaas-2BS and QCL.zaas-3BS were derived from 'Zhoumai 16', whereas those of QCL.zaas-7DL were from 'Gaocheng 8901'. Among these, QCL.zaas-1AL, QCL.zaas-2BS and QCL.zaas-7DL were newly reported loci, while QCL.zaas-3BS was located in close proximity to previously reported QTL. Eleven candidate genes were further identified, involving functional categories such as cytoskeletal formation, auxin homeostasis and polar transport, transcriptional regulation, energy metabolism, membrane lipid composition, and light signal perception. Moreover, 4 kompetitive allele specific PCR (KASP) markers were developed and validated in a natural panel of 130 wheat varieties. Among them, Kasp-CL-1AL and Kasp-CL-2BS exhibited significant allelic effects (P<0.05) in the natural population, serving as candidate tools for marker-assisted selection of coleoptile length. This study provides novel genetic loci and available KASP markers for genetic improvement of coleoptile length and breeding for deep-sowing tolerance and drought resistance in wheat.
王丽娜, 张倩, 刘金栋, 李楠楠, 吕永军, 邹少奎, 李顺成, 杜晓宇, 于海飞, 李永刚, 黄智博, 韩玉林. 普通小麦胚芽鞘长度的全基因组连锁分析及育种标记开发[J]. 农业生物技术学报, 2026, 34(11): 2292-2302.
WANG Li-Na, ZHANG Qian, LIU Jin-Dong, LI Nan-Nan, LYU Yong-Jun, ZOU Shao-Kui, LI Shun-Cheng, DU Xiao-Yu, YU Hai-Fei, LI Yong-Gang, HUANG Zhi-Bo, HAN Yu-Lin. Genome-wide Linkage Analysis and Breeding Marker Development for Coleoptile Length in Common Wheat (Triticum aestivum). 农业生物技术学报, 2026, 34(11): 2292-2302.
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