Oleate Promotes Adipogenesis by Activating the PPARγ in Bovine (Bos taurus) Stromal Vascular Fraction Cells
ZHENG Yan1, MA Zheng1, WANG Xiao-Yu1, QIU Ju1, ZHANG Yu1, ZAN Lin-Sen1,2,3, LI An-Ning1,2,3,*
1 College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2 National Beef Cattle Improvement Center, Yangling 712100, China; 3 Shaanxi Modern Cattle Industry Engineering Research Center, Yangling 712100, China
Abstract：Oleate has a significant promoting effect on adipogenesis, however, the exact mechanism of action is not clear. In order to investigate the effect of the addition of exogenous oleic acid on adipogenesis of the bovine (Bos taurus) stromal vascular fraction (SVF) cells and its specific mode of action, oleate was found to significantly promote adipogenesis by Oil Red O staining in this study. The results of qPCR and Western blot detection showed that, compared with the control group, the expression of adipogenic differentiation markers genes—peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) showed no significant changes in mRNA and protein levels, but the expression of lipogenic genes downstream of PPARγ, fatty acid-binding protein 4 (FABP4), diacylglycerol acyltransferase 2 (DGAT2), fatty acid transport protein 1 (FATP1), and perilipin 1 (PLIN1), was significantly increased at both mRNA and protein levels. PPARγ is a ligand-activated transcription factor, Therefore, oleate was speculated to serve as a ligand for PPARγ. The polarization value (mP) was found to decrease with increasing oleate concentration by ligand competitive binding assay, with a trend similar to that of the positive control rosiglitazone. The above results suggested that oleate acted as a ligand to activate PPARγ, which in turn promoted adipogenesis. This study provides some theoretical basis for the study of high-grade marbleized beef.
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