Differential microRNA Analysis of Response to Botrytis cinerea Infection in Grapes (Vitis vinifera) and Screening of Genes Related to Botrytis cinerea Resistance
NIU Li-Li6,*, ZHOU Hui1,*, WU Yun-Long1, HAN Ze-Yun1, CAO Hong-Yan2, DU Ting-Ting2, LIU Li-Yuan1, XI Lin4, MENG Dong2, YANG Qing2, SONG Yang-Bo3,**, HE Kun-Zu5,7,**
1 Turpan Grape Industry Development Promotion Center, Turpan 838000, China; 2 College of Forestry, Beijing Forestry University, Beijing 100083, China; 3 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China; 4 University of Hohenheim, Stuttgart 70599,Germany; 5 Turpan Silu Mingzhu Agricultural Biotechnology Co., Ltd., Turpan 838000, China; 6 Institute of Medicinal Plant Development, China Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; 7 Tianjin University of Science and Technology, Tianjin 300222, China
Abstract：MicroRNA (miRNA) mediated post transcriptional regulation plays a crucial role in plant responses to pathogens. In order to screen miRNAs in grapes (Vitis vinifera) that respond to Botrytis cinerea infection, this study infected grape leaves with B. cinerea spore suspension and constructed a small RNA library for high-throughput sequencing. A total of 171 known miRNAs and 251 new miRNAs were obtained in response to B. cinerea, of which 53 miRNAs showed differential expression after inoculation, especially miRNA399a, miRNA399b, and miRNA399h belonging to the miRNA399 family, which showed significant downregulation. The susceptibility of grapes to B. cinerea was determined by analyzing the phenotype, lesion area, and pathogenicity of leaves. 8 key target genes were obtained by predicting the downstream target genes of the 3 key miRNAs mentioned above. qPCR analysis showed that the target genes of miRNA399b and miRNA399h were significantly upregulated when infected with B. cinerea. This study provides a theoretical basis for further understanding the resistance response of fruit trees to B. cinerea during domestication and for breeding resistant grape varieties.
牛丽丽, 周慧, 武云龙, 韩泽云, 曹红燕, 杜婷婷, 刘丽媛, 郗琳, 孟冬, 杨清, 宋洋波, 何坤祖. 葡萄响应灰霉菌侵染的差异microRNA分析及抗灰霉菌相关基因的筛选[J]. 农业生物技术学报, 2025, 33(2): 303-315.
NIU Li-Li, ZHOU Hui, WU Yun-Long, HAN Ze-Yun, CAO Hong-Yan, DU Ting-Ting, LIU Li-Yuan, XI Lin, MENG Dong, YANG Qing, SONG Yang-Bo, HE Kun-Zu. Differential microRNA Analysis of Response to Botrytis cinerea Infection in Grapes (Vitis vinifera) and Screening of Genes Related to Botrytis cinerea Resistance. 农业生物技术学报, 2025, 33(2): 303-315.
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