Isolation and Identification of Endophytic Bacteria from Panax notoginseng and Screening of Strains with Antibacterial Functions
MA Shuang, QIAO Yan-Fang, DONG Yan-Qun, LI Yun-Qian, BI Yong-Ning, ZHANG An-Qi, ZHANG Tie, XIONG Gao, CHEN Zhong-Jian, WANG Yong, SUN Yu-Qin, WANG Teng*
Wenshan Sanqi Research Institute, Wenshan University, Wenshan 663099, China
Abstract：Panax notoginseng is a renowned Chinese medicinal plant. Phytophthora blight, incited by Phytophthora cactorum, represents one of the most devastating diseases affecting its cultivation, causing significant losses in both yield and quality. To screen for novel microbial resources for the environmentally friendly control of Pa. notoginseng blight, this study utilized healthy Pa. notoginseng plants from disease-affected fields. Samples from 6 parts(main root, lateral root, stem, leaf, petiole, and fruit) were collected. Endophytic bacteria were isolated using the dilution spread plate method. The isolates were identified based on morphological characteristics and 16S rRNA gene sequencing. The ultimate aim was to screen for biocontrol strains with potent inhibitory efficacy against the pathogen causing Pa. notoginseng blight. The plate confrontation method was used to determine the antagonistic ability of all strains against Pa. notoginseng phytophthora blight, and the optimal antagonistic strain was selected for pot experiments to verify its control effect. A total of 58 isolates were obtained from the healthy Pa. notoginseng plants in the phytophthora blight fields. Through the plate confrontation method, 13 antagonistic bacteria with significant inhibitory effects on Ph. cactorum were screened out. Among them, strain PNMR-1(identified as Serratia plymuthica) isolated from taproots showed the best antagonistic effect, with an inhibition rate of 60.56% after 7 d of confrontation culture. Observation under an optical microscope revealed that this strain could significantly inhibit the mycelial growth of Ph. cactorum and cause mycelial deformation. The results of pot experiments indicated that the control effect of strain PNMR-1 on Pa. notoginseng phytophthora blight was 67.54%, which was slightly lower than that of the chemical fungicide fluazinam treatment(70.19%), but the difference was not significant. This study isolated a new biocontrol strain, S. plymuthica PNMR-1, which had a significant inhibitory effect on the pathogen of Pa. notoginseng phytophthora blight, this strain can be used as an excellent microbial resource for the biological control of Pa. notoginseng phytophthora blight.
马爽, 乔燕芳, 董砚群, 李云倩, 毕咏凝, 张安琪, 张铁, 熊高, 陈中坚, 王勇, 孙玉琴, 王腾. 三七内生细菌的分离、鉴定及其抗菌功能菌株筛选[J]. 农业生物技术学报, 2026, 34(8): 1816-1824.
MA Shuang, QIAO Yan-Fang, DONG Yan-Qun, LI Yun-Qian, BI Yong-Ning, ZHANG An-Qi, ZHANG Tie, XIONG Gao, CHEN Zhong-Jian, WANG Yong, SUN Yu-Qin, WANG Teng. Isolation and Identification of Endophytic Bacteria from Panax notoginseng and Screening of Strains with Antibacterial Functions. 农业生物技术学报, 2026, 34(8): 1816-1824.
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