Construction of Recombinant Lactococcus lactis Expressing IFN-γ and Evaluation of Its Clinical Efficacy
WANG Yu-Qi1, SUN Pei-Wen1, WANG Wei1, PANG Kun1, WANG Yu-Yu1, LYU Chang-Rong1, SUN Wen-Yang2, LIANG Xiao-Jun3, LIN Yong-Kang4, HUA Jin-Lian1, MU Hai-Long5,*, LI Na1,*
1 College of Animal Medicine/Shaanxi Stem Cell Engineering & Technology Center, Northwest A&F University, Yangling 721000, China; 2 Ningxia Vocational College, Yinchuan 750021, China; 3 Animal Science Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021, China; 4 Qingdao Runqian Bioengineering Co., Ltd., Qingdao 266000, China; 5 Medical Research and Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712000, China
Abstract：Interferon-γ (IFN-γ) exhibits broad-spectrum antiviral activity and plays an important role in animal husbandry. This study aimed to construct a recombinant lactic acid bacterium expressing IFN-γ and evaluate its safety and therapeutic effect in clinical diarrheal diseases. Through molecular cloning technology, the goat IFN-γ gene was successfully inserted into the target plasmid pNZ8149, and the recombinant plasmid pNZ8149-IFN-γ was constructed. Furthermore, the recombinant Lactococcus lactis pNZ8149-IFN-γ/NZ3900 (rL. lactis-IFN-γ) was successfully constructed. Methods such as restriction enzyme digestion identification, bacterial liquid PCR, and DNA sequencing were used to verify the correctness of the recombinant plasmid, and it was identified by Western blot that the recombinant strain could successfully express IFN-γ. The recombinant probiotic was evaluated for safety in animals such as cat (Felis silvestris catus) and goat (Capra hircus), and the results showed that the recombinant strain had no obvious toxicity to the experimental animals. The effect of the recombinant probiotic preparation was evaluated through naturally diseased animals (calves (Bos taurus) with persistent diarrhea, forest musk deer (Moschus berezovskii) with diarrhea, and dogs (Canis lupus familiaris) with gastroenteritis). The results showed that the recombinant probiotic preparation had a significant effect on the treatment of diarrhea in different species. The results of this study indicated that the recombinant lactic acid bacterium expressing IFN-γ could not only be safely applied in clinical practice, but also had a good effect in the treatment of animal diarrheal diseases. This study provides a new treatment strategy for animal diarrheal diseases and other related diseases caused by viral infections.
王瑜琪, 孙培文, 王威, 庞坤, 王煜煜, 吕长荣, 孙文阳, 梁小军, 林永康, 华进联, 穆海龙, 李娜. 表达IFN-γ的重组乳酸乳球菌的构建及其临床效果评价[J]. 农业生物技术学报, 2026, 34(2): 431-443.
WANG Yu-Qi, SUN Pei-Wen, WANG Wei, PANG Kun, WANG Yu-Yu, LYU Chang-Rong, SUN Wen-Yang, LIANG Xiao-Jun, LIN Yong-Kang, HUA Jin-Lian, MU Hai-Long, LI Na. Construction of Recombinant Lactococcus lactis Expressing IFN-γ and Evaluation of Its Clinical Efficacy. 农业生物技术学报, 2026, 34(2): 431-443.
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