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Abstract To research the effect of breeds and feeding patterns on chicken meat flavor, and screening of inosine monophosphate acid (IMP) and intramuscular fat (IMF) candidate genes, Chengkou chicken、Daninghe chicken、Qingjiaoma chicken were selected as the study populations, to analyze IMP content in breast muscle by high performance liquid chromatograph, extrac the IMF in breast muscle by soxhlet extraction. The expressions of 5 candidate genes related to IMP and 9 candidate genes related to IMF were detected with Real-time quantitative PCR. The results revealed that the IMP content of Chengkou chicken was higher than that of Daninghe and Qingjiaoma chickens, the IMP content of scatter-feed chicken was higher than that of henhouse-feed; the IMF content of Chengkou chicken was lower than that of Daninghe and Qingjiaoma chickens, the IMF content of scatter-feed chicken was lower than that of henhouse-feed. The expression levels of IMP candidate genes, aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase (PURH) and adenosine monophosphate deaminase1 (AMPD1), and the IMF candidate genes, heart fatty acid binding protein (H-FABP), leptin receptor (LEPR), fatty acid transport protein 1 (FATP1) and uncoupling protein 3 (UCP3) were correlated with the contents of IMP and IMF in breast muscle, respectively. The results showed that both breed and feeding pattern had significant influence on IMP, IMF contents and expression of their candidate genes. The expression levels of candidate genes were closely related with IMP and IMF content. The results of this research will provide some theoretical basis for high quality poultry breeding.
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Received: 24 February 2014
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GONG Xue-Fang, XIE Shou-An, YANG Ping, HU Hong-Yan, GUO Li, CHE Xian-Rong, CHEN Di, WANG Yan-Lai, LV Shu-Jie. Preliminary Screening of Differentially Expressed Genes of Female and Male Antenna of Agrilus zanthoxylumi[J]. 农业生物技术学报, 2019, 27(7): 1233-1245. |
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