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| Genetic Diversity Analysis on the Main Chinese Cultivated Varieties of 6 Cotton Populations with ISSR Markers |
| He Jue-Min 何觉民 |
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Abstract Abstract: In the present paper, the genetic diversity 6 kind of 48 cotton trains from different origins were estimated using inter simple sequence repeat (ISSR) markers. 11 among 60 ISSR primers were chosen for their reproducibility and high polymorphism. a total of 90 fragments were amplified, in which the percentage of polymorphic loci (PPL) was76.1%, Results of POPGENE analysis indicated that genetic diversity of Xinjiang population was the highest (PPB= 91.43%, H= 0.3769, I= 0.5464),and Zhongmiansuo, Henan population followed it and genetic diversity was near among other three population. Analysis of Nei’s genetic diversity index indicated that the intraspecific genetic diversity were 82.42% ,diversity among populations were 19.72%; the gene differentiation (Gst) was 0.1759 and the gene flow (Nm) was 2.34 ;Result of UPGMA cluster analysis indicated that the relationship between Shandong and Shanxi is nearest It were the first clustered with Hebei, and Henan and CCAS were followed successively, Xinjiang population were clustered finally.
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Received: 06 July 2007
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