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Abstract A cDNA library was constructed using fibers of Gossypium hirsutum 7235 line. The library was randomly sequenced and an expressed sequence tag (EST) with the function of lipase was obtained. The full-length cDNA encoding lipase in 7235 line was cloned by the rapid amplification of cDNA ends (RACE) technique and named GhLipase. Sequence analysis showed that the full-length cDNA was 1 286 bp which contained a major open reading frame of 368 amino acids, 5′ un-translated regions, 3′ un-translated regions, and a Poly (A) tail. The sequence was submitted to GenBank, and accession No.EU273298. It had high homology to reported lipase genes from other plants. RT-PCR analysis confirmed that the GhLipase gene was up-regulated in ovules and fibers compared with roots, stems, and leaves of 7235. Southern blot analysis showed that there were two copies of the GhLipase gene in the genome of G. hirsutum. GhLipase gene was mapped on chromosome A13 in [(TM-1 × Hai7124) TM-1] population.
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Received: 03 November 2007
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