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Abstract PCR-SSCP method was used to identify the polymorphism sites in exon 2 domain of ADSL gene and 5’-flanking domain of GARS-AIRS-GART gene. Association between the founded polymorphism sites as well as pyramiding genotypes and Inosine Monophosphate (IMP ) contents in muscles were tested by least square analysis. The reproduction performance of individuals with different pyramiding genotypes were also analyzed. The results showed the founded polymorphism sites C3484T and C-179T had significant correlation with IMP contents in muscles. The TTs were two advantageous genotypes. Their additive effects for ADSL gene and GARS-AIRS-GART gene were respectively 0.1710 mg/g and 0.128 mg/g, and the dominant effects were -0.0250 mg/g and 0.031 mg/g. The pyramiding genotype TTTT had higher IMP contents than other pyramiding genotypes. The effects of two advantageous TTs in pyramiding genotype TTTT were still significant on IMP contents, however, their interaction effect was not significant (P>0.05). The average weights of 90 days and egg reproduction in pyramiding TTTT populations had no variation.
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Received: 09 September 2008
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