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Abstract This study aimed to isolate and clone root-specific promoters in rice and provide resources for breeding. Integrating of our lab's database, a root specific gene(TIGR Locus: Loc_Os05g19570) was obtained after RT-PCR. A promoter named P12 with 1 950 bp in length of the gene was cloned by PCR from the genomic DNA of MH63. The cloned promoter was fused with gus gene to construct expression vector, and then introduced into rice (Oryza sativa ssp. japonica) Zhonghua11 by Agrobacterium-mediated transformation. The result of GUS histochemical staining and quantitative analysis showed that the promoter had root specificity and time difference. Also, 5' end different length deletions of P12 were fused with gus gene and were transformed into rice, respectively. Quantitative analysis of GUS activity showed the expression level of gus gene in root was badly reduced after deletion the region of －1 059~－819 bp, it displayed some cis-elements maybe presence in this region. A root-specific promoter was cloned in this study.
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Received: 27 April 2010
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