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| Cloning and Functional Analysis of the Regulative Sequence of Rice rbcS Gene |
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Abstract The regulative sequence (Posrbcs) of ribulose-1 ,5-bisphosphate carboxylase small subunit genes ( rbcS ) of rice was cloned by PCR amplification. The cloned Posrbcs was fused with gus gene, and then introduced into rice by Agrogacterium-mediated transformation. The result of GUS histochemical staining showed that gus gene driven by Posrbcs expressed in leaf , culm, sheath and glume, and not in endosperm. Also, 5’ end difference length deletions of Posrbcs were fused with gus gene and the fused genes transformed rice respectively. Quantitative analysis of GUS activity showed that the expression level of gus gene was reduced with the deletion of promoter. The result of this experiment also showed that light induction could enhance GUS activity. Furthermore, the deletion of light inducible elements such as I box, GT1binding factors, GATA box, T box could reduce GUS activity and postpone the light-induction time. Electrophoretic mobility shift assays showed I box, GT1 factor, T box and GATA box could bind with the nuclear protein.
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Received: 17 April 2006
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