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Abstract Activation tagging is very important in plant genomics study. In this paper, an activation tagging library containing independent 30000 Basta-resistance lines was constructed by Agrobacterium tumefaciens-mediated transformation which used Arabidopsis bzr1-D as materials. Among these transformants, 47 lines showed obvious phenotype which is different from bzr1-D, containing late flowering time、dwarf、different shape of leaf、longer leaf petiole、trichomeless leaf 、sterile and no kink between stem/leaf. some T-DNA flanking sequences were obtained by Tail-PCR and Nested PCR which is the base for cloning mutant gene and co-segregation analysis.
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Received: 26 June 2007
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