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| Mapping the Key Gene of Fruit Maturity Date in Peach[Prunus persica (L.) Batsch] by SSR markers |
| 2, 2, 2, 2 |
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Abstract As the model plant in the fruit trees, a number of important quality trait locus has been mapped in peach. Fruit maturity date is an important economic trait in peach. In this study, the self-crossed progenies of peach(Prunus persica (L.) Okubo) were used as material to mapping the maturity genes in peach. 165 SSR primer pairs from the genome of Prunus species were screened by bulked segregant analysis (BSA). Results showed that three primer pairs were related with maturity genes: dominant marker UDP96-003, and co-dominant markers BPPCT015, UDP97-402. The electrophoresis results of these three markers in random samples were analyzed by FsLinkageMap 2.0 and variance analysis for single marker, the result indicated that the rank of the three markers was UDP96-003, UDP97-402 and BPPCT015 with the genetic distance of 21.0 and 13.2 cM, respectively, and all of the three markers were linked with maturity gene. The analysis results from FsQtlMap showed that there was a major-QTL loci between BPPCT015 and UDP97-402 with the LOD of 13.35 and the effect value of 0.623, respectively, and the genetic values were qq :84.59 d, Qq:100.08 d and QQ:115.25 d, respectively. The single-gene effect value of the loci approximately 15 d. The results provide experimental evidence to previous inference, and the locus is anchored between the two SSR markers.
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Received: 14 December 2011
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