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| Cloning and Expression of an Ogura Cytoplasmic Male Sterile (OguCMS)-related MYB Transcription Factor in Brassica oleracea var. capitata |
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Abstract Ogura cytoplasmic male sterile(OguCMS) is the most widely used male sterile type in cabbage breeding. MYB transcription factors play a key role in regulation of plant defense response and multiple development processes. In present experiment, a R2R3-MYB transcription factor which down regulated 10.3 times in cabbage(Brassica oleracea var. capitata) OguCMS lines was cloned by SMART RACE strategy.The full-length cDNA of BOMYB1 was 1 141 bp, which contained a 196 bp long 5' untranslated region, a 246 bp long 3' untranslated region and a 699 bp long open reading frame(GenBank accession number: JN703995). It was localized in the nucleus by subcellular localization prediction. It was an anther preferentially expressed gene in cabbage, which reached its expression peak in the late development. It was induced by the regulation of plant hormones salicylic acid(SA) and jasmonate methyl (JA-ME), and consequently regulated the expression of anther development genes. The experimental results suggests that BOMYB1 may be one of the important genes which involved in OguCMS anther development.
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Received: 18 October 2011
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