|
|
|
| Screening and Characterization of Monoclonal Antibody to Bursaphelenchus xylophilus |
| JIANG Li-qin;ZHENG Jing-wu;CHEN Zheng-xian;WU Jian-xiang |
| Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China |
|
|
|
|
Abstract The pine wood nematode, Bursaphelenchus xylophilus, is one of the most important pathogens in pine trees. Exact, quick and sensitive method for identification and diagnosis of the nematode is the precondition for managing the nematode transmission. One hybrid cell lines, named as 1D3, secreting monoclonal antibody ( Mab ) to Bursaphelenchus xylophilus were developed by fusing splenocytes of BALB/c mice to mouse mycloma cell line SP2/0. The ELISA titer was 1∶5.12×105. This specific Mab was identified as IgG1.Western blotting analysis showed that the Mab could react with B. xylophilus. The production of monoclonal antibody to B.xylophilus provides a rapid and sensitive method for identification and diagnosis of the nematode.
|
|
Received: 03 June 2005
|
|
|
|
Corresponding Authors:
ZHENG Jing-wu
|
|
|
|
|
|
|
