Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究论文 Current Issue | Archive | Adv Search |
Screening and Characterization of Monoclonal Antibody to Bursaphelenchus xylophilus
JIANG Li-qin;ZHENG Jing-wu;CHEN Zheng-xian;WU Jian-xiang
Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
Download: PDF (533 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  The pine wood nematode, Bursaphelenchus xylophilus, is one of the most important pathogens in pine trees. Exact, quick and sensitive method for identification and diagnosis of the nematode is the precondition for managing the nematode transmission. One hybrid cell lines, named as 1D3, secreting monoclonal antibody ( Mab ) to Bursaphelenchus xylophilus were developed by fusing splenocytes of BALB/c mice to mouse mycloma cell line SP2/0. The ELISA titer was 1∶5.12×105. This specific Mab was identified as IgG1.Western blotting analysis showed that the Mab could react with B. xylophilus. The production of monoclonal antibody to B.xylophilus provides a rapid and sensitive method for identification and diagnosis of the nematode.
Key wordsBursaphelenchus xylophilus      monoclonal antibody     
Received: 03 June 2005     
ZTFLH:  S188  
Corresponding Authors: ZHENG Jing-wu   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
JIANG Li-qin
ZHENG Jing-wu
CHEN Zheng-xian
WU Jian-xiang
Cite this article:   
JIANG Li-qin,ZHENG Jing-wu,CHEN Zheng-xian, et al. Screening and Characterization of Monoclonal Antibody to Bursaphelenchus xylophilus[J]. , 2006, 14(3): 412-415.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2006/V14/I3/412
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech