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Preparation of Rubisco Antibody and Determination of Rubisco Content
of Flag Leaves in Wheat by ELISA |
| LI Wei-fang ;WANG Xiu-hai;HE Wen-juan |
| School of life sciences, University of Science & Technology of China, Hefei 230026, China |
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Abstract The ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco) is crucial to the overall modulation of photosynthesis. Rubisco was purified by (NH4)2SO4, DEAE-Sepharose F F and Sephacryl S 300 from wheat (Triticum aestivum L. cv.) leaves. The rabbit polyclonal antibody was prepared by vaccinating rabbit with purified Rubisco. The Rubisco antibody was showed specificity to Rubisco by Western blot. And using Rubisco antibody，contents variation of Rubisco in flag leaves was determined by ELISA. The result showed that Rubisco contents were positive relative with photosynthetic rates during development stages of flag leaves. This method is quicker and more accurate than that of immune precipitation.
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Received: 27 May 2005
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Corresponding Authors:
LI Wei-fang
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