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| Expression of vip2A(c) Gene from Bacillus thuringiensis in Insect Cell |
| SHI Yong-xia;LV Lei;XU Wei;YUAN Mei-jin;PANG Yi |
| State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China |
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Abstract The recombinant Bac-gv2 DNA was obtained by inserting a fused gfp gene with Bacillus thuringiensis vip2A(c) gene encoding possible enzymatic component under the control of the polyhedrin gene promoter of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Trichoplusia ni cell line TnHi5 was transfected with Bac-gfp and Bac-gv2 DNAs respectively. Fluorescent cells expressing the fusion protein GV2 were much fewer than those expressing GFP alone, and did not obviously increase in number from 2 to 5 days after transfection. This results show that Vip2A fusion protein may have an ADP-ribosylating activity on cell skeleton actin and exert an influence on insect cell, the production and diffusion of the budded virus.
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Received: 28 May 2005
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Corresponding Authors:
PANG Yi
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