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| To Transfer Anti-herbicide Gene into Upland Rice (Oryza sativa)Cultivars |
| La Honggui1 Wang Huaqi1** Huang Danian2 Hua Zhihua2 Yan Meixian2 Gao Zhenyu2 Feng Xiujing1 |
| (1.Center of Upland Rice, College of Agronomy and Biotechnology, China Agricultural University，Beijing Key Laboratory of Crops Genetic Improvement, Beijing 100094, China; 2.China National Rice Research Institute, Hangzhou 310006, China) |
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Abstract Abstract: The anti-herbicide gene bar was transferred into immature embryos calli from elite cultivars of upland rice（Oryza sativa L. ）297, 10 and so on with help of particle bombardment. Some regeneration plantlets were acquired after two selections on media containing herbicide Basta and differentiation. Further molecular test by both PCR amplification and Southern blotting and experiments in fields demonstrated that extrogenous gene bar was integrated into T0 genomes and inherited to T1. The tests of induction and differentiation media indicated that MB and MS were better for induction of calli and the modified media of RMB2 and RMS2 promoted differentiation frequency of calli remarkably. The obtained transgenic plants and established genetic transformation system of the experiment were available for further anti-herbicide molecular breeding and other genetic transformation.
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Received: 01 January 1900
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Corresponding Authors:
Wang Huaqi
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