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| Establishment of Genetic Transformation System on Chinese zoysia（Zoysia sinica Hance）via Agrobacterium tumefaciens Soaked Seeds |
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Abstract In this study, a high-frequency transformation system has been established by Agrobacterium tumefaciens soaked seeds transformation technology, Chinese zoysia germinating seed were used as transformation receptor. External factors that affect the efficiency of genetic transformation were studied and optimized, on the base of NPTⅡ. The major results as followed: seed was germinated for 7 days, then was infected with Agrobacterium which OD600 was 0.9 with 100μmol.L-1 AS for 48 hours after pumping 5min+30s vacuum treatment. Then co-cultivation for 3 days, selected the transferred plant by 75mg.L-1 kanamycin. It was originally proved by the PCR and GUS examination that NPT-Ⅱand GUS gene had been transferred into the genome. The positive transformation rate was 55.21% by the analysis of PCR.
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Received: 31 October 2008
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