Abstract The preparation condition of probiotics-loaded montmorillonite integrated the probiotics and crude silicate montmorillonite was optimized and its influencing factors research was also discussed. The results showed that number of probiotics attached to montmorillonite was invariablenes at montmorillonite weight of 0.5 g and increased with the initial concentration and volume of probiotics. The adsorption ability decreased with the increasing pH values and presented the same trend in different ionic concentration. At different temperature, the adsorption rate of montmorillonite to probiotics increased firstly and then decreased and the highest adsorption percent was up to 89.74% at 30℃. This research indicated that the strains adsorption depended on not only the biochemical and surface characters, but also the environmental condition such as temperature.
