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| Construction of recombianant expression vector of Giant Panda (Ailuropoda melanoleuca) growth hormone Gene and Its Expression |
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Abstract Medicine produced by transgenic microorganisms and plants would have the potential to change the traditional means of production, and to reduce the cost of medicine production. In this paper, a growth hormonegene (AmGH) from giant panda (Ailuropoda melanoleuca) was cloned, and pCAMBIA13011-GH, the plant expressing vector consisting of Giant Panda (Ailuropoda melanoleuca) growth hormone gene was constructed, which contains a CaMV35S promoter and a hygromycin resistance gene, and then transformed AmGH into tobacco leaf-disc by Agrobacterium-mediated method. A total of 50 regenerated plants with hygromycin resistance were obtained in the selection media. The 12 putative transgenic individuals were tested and 5of them were verified the presence and integration of the AmGH into the nuclear genome of tobacco plants by GUS, PCR analyses. Expression of AmGH gene has been confirmed in E coli and in plants. To our knowledge, this is the first report of the expression of Ailuropoda melanoleuca growth hormone gene (AmGH) in E coli and plant system, suggesting a major step in the production of plant-based for Ailuropoda melanoleuca growth hormone (AmGH)
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Received: 01 June 2006
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