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Abstract The reproductive function of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) oocytes reconstructed by the nuclei of first polar body (abbreviated to PbI) was explored in these experiments, respectively. Cumulus oocyte complexes (COCs) from the mouses were collected after superovulation, then PbIs were obtained from the COCs by 2% pronase treatment. The survival PbIs under different temperature was identified and stapled by the methods of morphology and trypanblau staining, later the contents including nuclei and a little cytoplasm of PbI was injected into the oocyte that its nuclei had been enucleated by micromanipulation before, the reconstructed oocytes by PbI was fertilized and cultured in vitro in order to observe its ability of further development. The results showed that vigor of PbIs was in the best status within 12 to 14 hours after superovulation, and still maintained during 48 hours under 4℃. 13 out of 117 reconstructed oocytes from Kunming and ICR mice developed into 2-cell embryos, and 3 out of 38 reconstructed oocytes from C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice was cleavage as 2-cell embryos. The experiments confirmed that the mouse oocytes reconstructed by the nuclei of PbI could be normally maintained its fertilization and further development, this has offered valuable reference for further utilization of gene resources for other mammalian animals.
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Received: 11 June 2007
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