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| Effects of donors’ sex and different cell treatments on in vitro development of yak-bovine interspecies nuclear transfer embryos |
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Abstract The purpose of this study was to evaluate the effects of donors’ sex, cell cycle synchromization treatments and donor nuclei obtained from fresh or frozen-thawed on in vitro developmental competence of yak-bovine interspecies nuclear transfer(NT) embryos. In this paper, we used bovine oocytes as recipient, yak ear fibroblast cell as donor. The results indicated that the development rate of male blastocysts was higher than female（56.6% vs 39.5％，P﹤0.05）, whereas cleavage and total cell number had no difference between the two groups. No significant difference was observed in the blastocysts development and quality of donor treat with serum starvation or confluence inhibition. There was no significant difference in embryos development of use fresh or frozen-thaw donor cell, whereas cleavage in group of fresh donor cell was significantly higher than frozen-thaw group（54.5％ vs 78.2％，P﹤0.05）. Our results demonstrated that donors’ sex could impact the in vitro developmental competence of yak-bovine interspecies NT embryos, whereas different cell cycle synchromization treatment and freezing had little influence.
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Received: 13 March 2007
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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