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| Study on the PCR-RFLP and Sequence Difference of Halothane Gene in Zang Pig |
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Abstract In this study, the part of halothane gene in 18149-18760 sites in 94 zang pigs was amplified by PCR. The sequence of PCR production is determined. The result shows that PCR production is 612 bp, and it included exon 17 and cDNA 1843 site. The result of HhaⅠpolymorphism analysis about this 612bp was all 94 zang pigs showed halothane masculine. Sequence analysis results indicated that two mutation sites are found in the sequences of the four zang pigs, and all mutation sites are transition and locate within introns in all 4 sequences. In intron 16 one site has transition heterozygote. The C1843→T1843 mutation site is not found in zang pigs.
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Received: 07 March 2007
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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