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Primary Analysis of Molecular Diversity in Populations of the
Fungus Ustilago scitaminea Syd. |
| QUE You-Xiong XU Li-Ping** ZOU Tian-Tang CHEN Ru-Kai |
(Key Laboratory of Eco-physiology & Genetics Improvement for Sugarcane, Ministry of Agriculture,
Fujian Agriculture and Forestry University, Fuzhou 350002, China) |
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Abstract Abstract: Eighteen isolates were obtained from sugarcane smut fungus (Ustilago scitaminea Syd). cultivated in six different provinces or regions including Fujian Province. On the basis of isolation of single spore and the acquisition of series of the isolates, 13 random primers of 10 bp were occupied in analysis of genetic diversity by RAPD. The largest dissimilarity coefficient was 0.89474, being observed in two isolates with codes of 7 and 9 originated from Fujian and Guangxi, respectively; the smallest value of 0.25217 was observed in two isolates originated in Fujian. Dendrogram of UPGMA cluster analysis revealed that 18 isolates of U. scitaminea Syd. were divided into 6 groups according to the dissimilarity coefficient of 0.75. Because of small dissimilarity coefficient, seven isolates obtained from sugarcane in Fujian Province belonged to two adjacent groups of cluster Ⅰ and cluster Ⅱ. High dissimilarity coefficient of 0.82353 was observed in two isolates collected from same host origin but with different geographical origin.The results of cluster suggested that the molecular diversity was associated with geographyic origins in some degree, but not related to host origin.
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Received: 01 January 1900
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Corresponding Authors:
XU Li-Ping
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