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Cloning and Sequencing of DNA Components of
Banana bunchy top virus Hainan Isolate |
| TIAN E ZHUANG Jun LIU Zhi-Xin* |
| (State Key Laboratory for Tropical Crop Biotechnology, Chinese Academy of Tropical Agriculture Science, Haikou 571101,China) |
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Abstract Abstract：Six DNA components of Banana bunchy top virus （BBTV）were amplified by PCR using total DNA from banana tissues which are of characteristic of typical BBTV symptom as template. The sequence analysis indicated that the 6 fragments were all full-length components of BBTV, they were 1 104, 1 067, 1 059, 1 045, 1 014 and 1 081 nt respectively. All of them have been submitted to GenBank(Accession No. AY450396, AY606084, AY494786, AY494788, AY606085 and AY494787, respectively). The viral sequences from different countries were compared. Results revealed that the sequence of component 1 was more conservative than other components, DNA1 of Hainan isolate shared homologies of 95.4% with that of Vietnam isolate, while both the sequences of component 2 and component 4 had more difference, and DNA2 of Hainan isolate only shared homologies of 83% with that of Guangzhou isolate. According to DNA1 sequence comparison results, the BBTV Hainan isolate should belong to Asia sub-group.
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Received: 01 January 1900
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Corresponding Authors:
LIU Zhi-Xin
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