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Cloning of Heart-type Fatty Acid-binding Protein (H-FABP ) Gene of Swine and
Difference of Its Expression at Different Stages |
| HUANG Yu-hai;WANG Yi-zhen;SHAN Ti-zhong;LIU Jian-xin;FENG Jie |
| Institute of Feed Science, Zhejiang University, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310029, China |
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Abstract Heart-type fatty acid-binding protein(H-FABP) gene mRNA extracted from muscle of pig(Duroc×Landrace×Yorkshire) was amplified using RT-PCR. And a DNA fragment of 211 bp in length was obtained and the PCR product was cloned into PGEM-T vector. The H-FABP gene was isolated and sequenced from the positive clones screened. Sequence analysis suggested that this fragment was partial sequence of H-FABP cDNA. The gene homology of fragment obtained in this study compared with that of reported H-FABP sequence in muscle of porcine was 99%. Based on the H-FABP gene clone, an optimal semi-quantitative RT-PCR method was successfully constructed. Using 18S rRNA as inner control, the difference of H-FABP gene expression at different stages of swine was researched. The result indicated that the H-FABP gene expression level decreased from 1 to 50 kg, and increased from 50 to 90 kg of body weight.
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Received: 20 October 2005
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Corresponding Authors:
ANG Yi-zhen
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