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| Construction and Analysis of Tamarix albif lonum M. T. Liu. Suppression Subtractive Hybridization Library Under Drought-Treated |
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Abstract A suppression subtractive hybridization (SSH) library was constructed from the tissue of Tamarix albif lonum M. T. Liu treated with PEG6000. The results showed that the rate of recombination in the library was 95% and the size of inserts was 100～1000 bp.100 ESTs drought stress-associated genes were obtained by sequencing the positive clones picked randomly. The EST expression profile showed that the resistance-related genes include osmotic regulator, signal transduction, transcriptional regulators, antioxidant enzyme, protein metabolism, transmembrane transport , ion homeostasis and photosynthesis. The results can contribute the further investigation of those genes in the future.
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Received: 30 March 2006
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