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Separation and In vitro Culture of the Goat Fetal Testicular Cells
and the Behavior of Male Germ-line Stem Cells(mGSCs) |
| DONG Wu-Zi1 HUA Jin-Lian1 ZHUANG Shu-Zhen1 SHEN Wen-Zheng1,2 DOU Zhong-Ying1** |
(1.Shaanxi Province Branch Center of National Stem Cell Engineering & Technology Center,
Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China;
2.Yangling Vocational and Technical College, Yangling 712100, China) |
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Abstract Abstract： The seminiferous tubules tissues from goat fetus were treated with 4 methods. The treatment with 0.1% colleagenase for 15 min and then with 0.25% trypsin for 5 min showed ideal cell dispersal degree after centrifugation and washing. The in vitro culture of the cells separated with different medium system showed that mulberry-shaped mGSCs clusters and a single layer of Sertoli cells appeared as original generation in 120 h, the mGSCs clusters developed half-suspendedly and distributed in different locations with the Sertoli cells in the plate; The cells in the margin area of incompletely digested mGSCs clusters and the single cells from them were obviously homogenized after culture for 5 days in fiber cell feeding layer from mice fetus, while same-treated mGSCs clusters co-cultured with Sertoli cells did not displayed clear homogenization in marginal cells of the clusters in the same culture system; And the first generation of mGSCs clusters co-cultured with Sertoli cells formed the same morphologic properties with the original generation of clusters, cells in clusters were tight and mGSCs divided slower than one in MEF feeding layer.
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Received: 01 January 1900
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Corresponding Authors:
DOU Zhong-Ying
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