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| Rat Adipocyte Apoptosis Induced by Tumor Necrosis Factor-α |
LIN Ya-Qiu LI Rui-Wen SUN Chao CHEN Guo-Zhu
YANG Yong-Qing MENG De-Lian YANG Gong-She** |
(Laboratory of Animal Fat Deposition and Musle Development, Northwest Sci-tech University
of Agriculture and Forestry，Yangling 712100，China) |
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Abstract Abstract: The effects of tumor necrosis factor-α(TNF-α) at different concentration levels on rat adipocyte apoptosis were detected by optical microscopy, agarose gel electrophoresis and flow cytometry methods. The morphologic changes of rat adipocyte apoptosis induced by TNF-α were correlated linearly with the concentrations of TNF-αwithin a range from 5 to 20 ng/mL, and the higher concentration of TNF-α could induce more obvious apoptosis. The morphologic changes of rat adipocytes treated by 5 ng/mL TNF-α were clearly shown, but their DNA ladders didn't appear by the DNA electrophoresis analysis, indicating that the morphologic changes occurred earlier than the biochemical changes. TNF-α induced the rat adipocyte apoptosis in a dose-dependent manner within a concentration range from 5 ng/mL to 20 ng/mL. Compared with the control, the apoptotic effect induced respectively by 5, 10, 15 and 20 ng/mL TNF-α was significantly difference (P<0.01), but the effect among 10, 15 and 20 ng/mL TNF-α treatments was not difference (P >0.05). Thus the optimum concentration of TNF-α inducing apoptosis was determined at the level of 10 ng/mL.
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Received: 01 January 1900
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Corresponding Authors:
YANG Gong-She
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