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| Establishment of Direct Adventitious Shoot Regeneration System and Genetic Transformation System of Euonymus fortunei var. Radicans Mediated by Agrobacterium tumefaciens |
| Shang Ai-Qin Zhao Tian |
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Abstract Using in vitro hypocotyls as explants, the adventitious shoots of Euonymus fortunei var. Radicans were differentiated directly from the basal MS medis supplemented with different plant growth regulators through organogenesis. The results showed that the highest regeneration frequency was obtained with MS medium containing 0.5 mgL-1 6-BA and 0.01 mgL-1 NAA. Ninety-two percent of regeneration frequency and 4.2 shoots per explants were obtained after 30 days of culture. The binary vector pBCGm containing Galanthus nivalis agglutinin (GNA) gene was introduced into Agrobacterium tumefaciens LBA4404. The hypocotyl segments of Euonymus fortunei var. Radicans were infected through Agrobacterium tumefaciens. The GNA gene was integrated into the genome of transgenic plants confirmed by PCR and PCR-Southern analysis. The highest transformation frenquency was obtained through the following transformation procedure after preculture for 0 d, the explants were infecter for 30 min with OD600=0.6 Agrobacterium tumefacien, and the co-cultivated for 3d.
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Received: 16 May 2007
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Corresponding Authors:
Zhao
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