Abstract Zwittermicin A (ZwA) is a novel antibiotic which shows diverse inhibitory activity against a broad target of plant pathogens. It was first discovered from Bacillus cereus strain UW85, and zmaR is its resistant gene. Since we have already got the entire genome of Bacillus thuringiensis strain YBT-1520, which is also produce ZwA, we analyze the synthesis gene cluster of ZwA in strain YBT-1520 and found that at the downsteam of the gene cluster there was some sequence highly similar with ABC transporter（zwa-FEG). When the zwa-FEG was transferred to E. coli DH10B, it could obtain the resistant ability to ZwA. When zwa-FEG gene was transferred to ZwA-producing strain UW85, the production of ZwA was improved. It suggested that zwa-EFG is a cluster coding ABC transporter, and it could affect the resistance to ZwA.
