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| AHL lactonase Increases ZwA’s suppression against soft rot disease in Bacillus cereus |
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Abstract Zwittermicin A (ZwA) is a broad spectrum, novel antibiotic which shows diverse inhibitory activity against a broad target of plant pathogens; and Acyl-homoserine Lactonase (AHL lactonase) can attenuate expression of virulence genes in bacterial pathogens and then to suppress certain plant diseases by degrading N-acyl-homoserine lactones (AHLs) which are involved in the inducement of the expression of many virulence genes in bacterial pathogens. In order to increase disease suppression by combining the two different disease suppression mechanisms of ZwA and AHL lactonase, AHL lactonase gene was introduced into ZwA+ Bacillus cereus strains, and the recombinant stains were attained and showed higher expression level of AHL lactonase compared to their parental strains, without affecting the expression level of ZwA. Furthermore, the recombinant stains exhibited stronger ability of AHLs inactivation and higher restraint to the potato rot disease caused by Erwinia carotovora than their parental stains. Thus, it was indicated it was feasible to increase disease suppression by combining the biological functions of ZwA and AHL lactonase.
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Received: 10 July 2006
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