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| Cloning and Expression Analysis of the Anthocyanin Transcriptional Activator Gene stmyc of Solanum tuberosum (potato) |
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Abstract Based on the conserved sequence of Perilla’s MYC mRNA gene from GenBank, the conserved fragment of MYC like anthocyanin transcriptional activator gene was amplified by using RT–PCR from the RNA of purple potato coat. Then the cDNA 3-end sequence was amplified by using 3’-Race technique from the RNA of purple potato coat. Sequence analysis show that the nucleotide sequence of this gene is 1106bp, containing a complete open reading frame and encoding 326aa, named stmyc. The deduced protein of stmyc gene shares 46.98％identity with MYC-GP protein. stmyc gene encodes a typical bHLH protein domain that is more important for MYC-like proteins. The results of RT-PCR expression analysis show that stmyc expressed in the leaves, stems, tuber skins, tuber fleshes and roots of purple potato and the leaves, tuber skins, tuber fleshes of white potato with the different expression levels. It is suggested that the stmyc is constitutive expressed in potato.
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Received: 11 December 2007
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