Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究简报 Current Issue | Archive | Adv Search |
Optimization of ISSR-PCR Reaction System in Yane geese
Download: PDF (347 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  Using the genomic DNA extracted from Yane geese, the orthogonal design was used to optimize ISSR-PCR amplification system in five levels of five factors(Taq DNA polymerase, Mg2+,DNA template, dNTPs and primer), respectively. Based on the preliminary experiment, the density within factors of ISSR-PCR was further optimized. The purpose of this experiment was to determine the reaction condition of ISSR-PCR fleetly and accurately, which was basis for the study on genetic diversity, map construction and gene localization of Yane geese. The results showed that a better amplification of ISSR was obtained with the reaction system containing 0.20umol/L primer, 0.28mmol/L dNTPs, 40ng templet DNA of Yane geese, 1.5mmol/L Mg2+,1.0U Taq DNA polymerase in the total volume of 25uL. Based on the orthogonal design experiment, the prime reaction condition of ISSR-PCR can be established through the density within factors of ISSR-PCR further optimized.
Received: 12 September 2007     
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
NULL
Cite this article:   
NULL. Optimization of ISSR-PCR Reaction System in Yane geese[J]. , 2009, 17(5): 0-.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2009/V17/I5/0
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech