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Abstract Abstract BALB/c mice were immunized intraperitoneally with purified recombinant mEIFN-protein. Murine myeloma cells were fused with the splenocytes of the immunized mice after the fourth immunization. An indirect ELISA with recombinant mEIFN-derived from the baculovirus expression system as antigen was used to screen antibody-producing hybridomas and 15 McAbs against mEIFN-were obtained. Twelve hybridomas cells of them can produce McAbs steadily after 3 cycles of subcloning. All the 12 McAbs were reactive in (indirect fluorescence assay) IFA tests, and all of them showed positive reaction to mEIFN-. Four McAbs are IgG1, two McAbs are IgG2a，three McAbs are IgG2b and three McAbs are IgM isotype, respectively. The light chains of all McAbs are chain. The seven McAbs of them showed positive reaction to GST-mEIFN-(1-84) protein, and the others show positive reaction to GST-mEIFN-(80-146) protein in specific ELISA tests, respectively. The McAbs against mEIFN- protein developed in this study would be useful as a basis of detection methods for equine IFN-, investigating the structure and biological effects of equine IFN-.
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Received: 26 March 2007
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