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| Construction and Primary Characterization of cDNA Library from Earthworm Induced with Cadmium |
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Abstract The acute toxicity of heavy metal Cd to earthworm (Eisenia fetida) was studied by the method of artificial soil test. It was shown that Eisenia fetida is tolerance specie which is applicable for accumulation indication. A cDNA library of Eisenia fetida was constructed .Total RNA was extracted from Eisenia fetida after induction with Cd2+ by Trizol regent. The full-long cDNA library was constructed successfully by using the SMART technique. The results showed that the primary titer of the constructed cDNA library was 3.02×105 pfu/mL, while that of the amplified library was 8.67×109 pfu/mL .The recombination rate was about 97.3%，and the length of most cDNA inserted in the library was about 300-3000bp.The library must be helpful to make clear the components of Eisenia fetida and establish the base for research into heavy metal detoxification and immune mechanisms at molecular level.
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Received: 23 November 2006
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