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Detection of Roundup Ready Soybean in Highly Processed Products by Triplex Nested PCR
ZHANG Ming-hui;GAO Xue-jun;YU Yan-bo;QIN Jun;LI Qing-zhang
Research Center of Life Science and Biotechnique, Detecting Center of Agricultural Genetically Modified Organism of Heilongjiang Province , Northeast Agricultural University, Harbin 150030, China
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Abstract  Seven representative highly processed product samples (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, crude soybean oil, soybean refine oil, soybean salad oil) were detected by the triplex nested PCR. The first triplex PCR could not  detect the insert signals in the processed products, and the sensitivity was 0.5%. However, the second triplex PCR could simultaneously detect RR soybean elements, lectin, 35S-CTP and EPSPS-NOS, and a sensitivity was 0.005%. The results indicate that triplex nested PCR is suited to qualitative detection for Roundup ready soybean in highly processed products.
Key words Roundup Ready soybean      highly processed products      triplex nested PCR      genetically modified organism detection     
Received: 30 August 2005     
ZTFLH:  S184  
  S188  
Corresponding Authors: GAO Xue-jun   
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ZHANG Ming-hui
GAO Xue-jun
YU Yan-bo
QIN Jun
LI Qing-zhang
Cite this article:   
ZHANG Ming-hui,GAO Xue-jun,YU Yan-bo, et al. Detection of Roundup Ready Soybean in Highly Processed Products by Triplex Nested PCR[J]. , 2006, 14(5): 752-756.
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http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2006/V14/I5/752
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