Research Center of Life Science and Biotechnique, Detecting Center of Agricultural Genetically Modified Organism of Heilongjiang Province , Northeast Agricultural University, Harbin 150030, China
Abstract Seven representative highly processed product samples (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, crude soybean oil, soybean refine oil, soybean salad oil) were detected by the triplex nested PCR. The first triplex PCR could not detect the insert signals in the processed products, and the sensitivity was 0.5％. However, the second triplex PCR could simultaneously detect RR soybean elements, lectin, 35S-CTP and EPSPS-NOS, and a sensitivity was 0.005%. The results indicate that triplex nested PCR is suited to qualitative detection for Roundup ready soybean in highly processed products.
