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| Effects of DNA Concentrations and Storage Conditions on PCR Detection of Transgenic Rice |
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Abstract The effects of DNA concentrations(0.2~16 ng/μL), transgene contents (50.0%~0.05%,W/W), storage temperatures(22℃，4℃，－20℃) and storage periods (1，2，3 weeks and 1, 3 months) on qualitative PCR detection of transgene from transgenic rice(Oryza sativa ssp.indica) were evaluated. The result showed that all the primers can amplified targeted fragment when DNA concentration was higher than 0.4 ng/μL. For mixed samples with 1.0% and 0.1% transgene, the lowest DNA concentration which was still detectable was 1 ng/μL and 2~4 ng/μL, respectively. Sample storage at the three tested different temperatures did not affect subsequent PCR detection. And the brightness of electrophoresis strips of PCR products were gradually weakened after 3 months storage of DNA sample.
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Received: 28 October 2009
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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