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| Screening and Identification of Upstream Transcription Factors of OfLCYB Gene from Osmanthus fragrans |
| XU Meng-Han1,*, QING Hong-Sheng2,*, QIAN Jie-Yu1, ZHANG Chao1,**, FU Jian-Xin1,** |
1 College of Landscape Architecture/Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang A&F University, Hangzhou 311300, China;
2 National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; |
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Abstract Carotenoids are important pigments that determine the coloration of Osmanthus fragrans petals, and lycopene β-cyclase (LCYB) is a key enzyme in the carotenoid synthesis pathway of O. fragrans. In this study, O. fragrans cultivar 'Yanhong Gui' was used as material, the bait vector of OfLCYB promoter and the yeast one-hybrid cDNA library of O. fragrans petals were constructed. The upstream transcription factor OfMYB308 interacting with OfLCYB promoter was screened by yeast one-hybrid. OfMYB308 had a typical EAR repressor motif and belonged to S4 subgroup of R2R3-MYB transcription factors. The expression level of OfLCYB was positively correlated with that of OfMYB308 gene, and both of them increased with flower opening. The results of yeast one-hybrid point-to-point verification and dual luciferase assay showed that OfMYB308 could bind to the OfLCYB promoter and significantly inhibit its transcriptional activity. This study provides theoretical basis for revealing the regulatory network of carotenoid synthesis in O. fragrans.
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Received: 25 April 2025
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Corresponding Authors:
** zhangc@zafu.edu.cn; fujx@zafu.edu.cn
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| About author:: *These authors contributed equally to this work |
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