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| NtCPS2 Gene Affects the Occurrence of Tobacco (Nicotiana tabacum) Glandular Hair by Regulating Gibberellin Synthesis |
| CAO Ke-Xin1, YANG Hui-Juan1, LIU Hua-Bing2, WU Jian2, CHEN Xiao-Xiang2, XU Shi-Xiao1,* |
1 Tobacco College, Henan Agricultural University, Zhengzhou 450002, China;
2 Zhejiang China Tobacco Industry Co., Ltd., Hangzhou 310008, China |
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Abstract The growth and development of foliar glandular hairs in tobacco (Nicotiana tabacum) is related to the aroma quality of tobacco leaves. In order to investigate the relationship between Ent-copalyl diphosphate synthase 2 (NtCPS2) and the growth and development of glandular hair and the synthesis of glandular hair secretion in tobacco, the wild-type strain (WT), NtCPS2 homozygous editing strain (KD) and overexpression strain (OE) of '8306' were used as materials to observe the phenotype of glandular hair and the density of glandular hair. Content of cyperane substances was determined and the expression of key synthetic genes was analyzed. The results showed that compared with WT, the density of leaf secretory glandular hairs in KD strains increased by about 75%, and the secretion of leaf glandular hairs increased significantly (P<0.05), while the secretion of leaf glandular hairs in OE strains was significantly lower than that of WT strains (P<0.05), and decreased by about 50% compared with WT strains (P<0.05). The expression level of cyclin B2 (NtCycB2) gene in the negative regulation of glandular hair gene was significantly lower than that in WT and KD strain, and the expression level of NtCycB2 gene in OE and KD strains showed an opposite trend, and the expression level of NtCycB2 gene was opposite to that of glandular hair phenotype. The gibberellin content of KD tobacco plant was significantly higher than that of WT strain (P<0.05). The gibberellin content of OE strains was significantly lower than that of WT strains (P<0.05). The content of gibberellin in KD strains increased, the density of secretory glandular hairs increased, and the content of cyperane substances, the main glandular hair secretions in the foliar strain increased. The OE strain showed an opposite trend to the KD strain. The results suggested that the NtCPS2 gene might promote the increase of gonadal hair density and foliar glandular hair secretion by positively regulating gibberellin biosynthesis. This study provides a new research direction for the regulation of the synthesis of tobacco glandular hair and tobacco aroma compounds by NtCPS2 gene through gibberellin pathway.
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Received: 22 May 2023
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Corresponding Authors:
* xushixiao@henau.edu.cn
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