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| Cloning, Bioinformatics Analysis of Transcription Factor NAC62 in Industrial Hemp (Cannabis sativa) and Its Response Analysis to Drought Stress |
| YANG Yu-Lei, ZHANG Han-Xue, WANG Shan-Shan, TANG Kai-Lei, DU Guang-Hui* |
| School of Agriculture, Yunnan University, Kunming 650500, China |
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Abstract Industrial hemp (Cannabis sativa) is an important economic crop, and its seed germination period is susceptible to drought stress, which ultimately has adverse effects on the growth and yield of hemp. NAC gene is a unique type of transcription factor in plants, which plays an essential role in plant response to abiotic stress. Based on the previous transcriptome data, a NAC gene from industrial hemp 'Yunma 1' was cloned by RT-PCR in this study, and named as CsNAC62 (GenBank No. XM_030652694). The results of sequence analysis found that the coding sequence of CsNAC62 gene contains 1 260 bp, encoding 419 amino acids, its protein molecular weight is 48.32 kD, and the isoelectric point is 8.24. The N-terminal of CsNAC62 contains a NAC domain composed of 150~160 amino acids. This domain is composed of 5 subdomains, which is a typical conservative domain of the NAM/NAC gene family. Phylogenetic analysis showed that CsNAC62 was closely related to the NAC3 protein of peach (Prunus persica) in all the tested species. The gene expression level of CsNAC62 in the germinated seeds of industrial hemp 'Yunma 1' gradually increased with the prolongation of the drought stress treatment (PEG-6000 simulation), and the expression level reached to the maximumon the 7 d of germinated seeds. In normal seed germination (control), the expression level of CsNAC62 increased slowly. These results indicated that the gene expression of CsNAC62 was induced by drought. This study preliminarily explored the expression mode of the CsNAC62 under PEG simulated drought stress, and provides a reference for further exploring its molecular mechanism of participating in the response to industrial cannabis drought stress.
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Received: 19 April 2023
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Corresponding Authors:
* dgh2012@ynu.edu.cn
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