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| Gene Identification and Expression Analysis of Histidine Phosphotransfer Protein AHP Family in Melon (Cucumis melo) |
| ZHAO Wang-Long, LI Jia-Qi, LI Meng*, WANG Ji-Qing, XIAO Huai-Juan, DU Qing-Jie, LI Juan-Qi |
| College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China |
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Abstract Arabidopsis histidine phosphotransfer proteins (AHPs) play important roles in plant growth, development and resistance to biotic or abiotic stresses. In this study, bioinformatics, transcriptome analysis and qRT-PCR were used to explore the biological function and expression pattern of AHP in melon (Cucumis melo), including the number of AHP gene family members, chromosome location, gene structure, subcellular localization, conserved motifs, phylogenetic analysis, cis-acting elements and gene expression. Results showed that the CmAHP family had 7 members, and distributed on 7 chromosomes. The length of amino acids ranged from 109 to 412 aa, all of which were hydrophilic. All the members were localized in the nucleus or extracellular matrix. Phylogenetic analysis revealed that the melon AHP members were highly homologous to cucumber (Cucumis sativus). CmAHPs promoter mainly contained cis-acting elements such as light response and hormone response elements. Gene expression analysis showed that CmAHP5 and CmAHP7 were highly expressed in all 5 tissues, including roots, leaves, female flowers, male flowers and fruits, which might play negative roles in fruit development and ripening. Transcriptome data showed that CmAHP5 and CmAHP7 might play negative and positive roles, respectively, in defending Fusarium wilt and powdery mildew. qRT-PCR data showed that CmAHP1~CmAHP5 were involved in low temperature response, among which CmAHP4 might be a key candidate gene. The present study provides a theoretical reference for analyzing the function of AHP gene in melon.
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Received: 16 September 2022
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Corresponding Authors:
* limengscience@163.com
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