藻螺共投对宽体金线蛭(<em>Whitmania pigra</em>)生长、消化、免疫和品质的影响

doi:10.3969/j.issn.1674-7968.2023.08.013

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Abstract The farming of Whitmania pigra was experiencing with the problems of single food type and food shortage supply, which limited the development of farming. Therefore, developing the various types of food for W. pigra was becoming urgent. The aim of the present study was to evaluate the effect of mixed feeding to W. pigra with Chlorella vulgaris and Sinotaia quadrata on growth, digestion, immune and pharmaceutic quality. The healthy W. pigra (initial weight (3.512±0.002) g) was divided into 3 groups randomly, with triplicates. They were feed with C. vulgaris individually (Algae group, CV), C. vulgaris and S. quadrata together (algae and snail mixed group, CV+SQ) as well as S. quadrata individually (snail group, SQ) for 30 d. The results showed that CV+SQ group obtained significantly higher final body weight (FBW), weight gain (WG), feed intake (FI) compared to other groups (P＜0.05), and specific growth rate (SGR) and feed conversion ratio (FCR) were significantly better than CV group (P＜0.05). For body composition, the leucine and lysine contents in CV+SQ group was significantly higher than those in SQ group (P＜0.05). The aspartic acid content was in opposite tendency. In addition, CV+SQ group exhibited significant higher α -amylase and antithrombin activities than other groups (P＜0.05), higher lipase and protease activities than CV group (P＜0.05), as well as higher superoxide dismutase (SOD) and catalase (CAT) activities than SQ group (P＜0.05). SDS-PAGE result indicated that the band of peptide with around 30 kD was in higher density in the CV+SQ group than in other groups. The transcription levels of insulin-like growth factors-1 (IGF-1), inorganic pyrophosphatase (PPase), α-glucosidase (α-GLU), SOD, alkaline phosphatase (AKP), CAT and hirudin (WP) in CV+SQ group were significantly higher than those in SQ group (P＜0.05). In conclusion, mixed feeding to W. pigra with C. vulgaris and S. quadrata promoted the growth, digestive capacity, pharmaceutic quality and immune capacity. This study provides a theoretical basis for optimizing feeding strategies of the farming of W. pigra.

Key words： Whitmania pigra Mixed feeding with algae and snail Growth Digestion Immunity Quality

Received: 15 September 2022

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Corresponding Authors: * gaoyol@163.com

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	HE Sheng-Sheng
	CHEN Zi-Yi
	YAN Jing-Nan
	WANG Ke-Hong
	GAO You-Ling

Cite this article:

HE Sheng-Sheng,CHEN Zi-Yi,YAN Jing-Nan, et al. Effect of Mixed Feeding with Algae and Snail on Growth, Digestion, Immune and Quality of Whitmania pigra[J]. 农业生物技术学报, 2023, 31(8): 1696-1709.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.08.013 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I8/1696

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