|
|
|
| Cloning, Tissue Expression of PTEN Gene in Muscovy Duck (Cairna moschata) and Its Localization in Follicles |
| LIN Jun-Yuan1, NIU Yu-Rui1, MEI Xiang1, CHEN Chu1, GUO Shun2, HU Zhi-Gang1, LIU Xiao-Lin1* |
1 College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
2 Animal Husbandry Development Center of Tongguan County, Tongguan 714300, China |
|
|
|
|
Abstract The phosphatase and tensin homologue deleted on chromosome 10 (PTEN) is a tumor suppressor gene. It plays an important role in cell growth, organ development and metabolism. In this study, the PTEN gene (GenBank No. OQ359981) was cloned using cDNA of Muscovy duck (Cairna moschata) ovary tissue as template, and bioinformatics analysis was performed. It was found that the full length of PTEN gene CDS was 1 212 bp, and the coded amino acid sequence was highly conserved. The expression of PTEN gene and protein in the kidney, liver, spleen, lung, heart, ovary and follicles of different grades were detected by qRT-PCR and Western blot. The results showed that PTEN was expressed in all the tissues, especially in ovary, spleen and heart; PTEN was expressed in both pre-hierarchical follicles and pre-ovulatory follicles, with the highest expression in small yellow follicles (SYF); Immunohistochemical staining confirmed that PTEN located in the granulosa cell layer of follicles. The above results suggest that the PTEN gene might be involved in the growth and development of duck follicles. The present study provides basic materials for further exploration of the regulation mechanism of PTEN gene in follicle development, and also provides new ideas for the study of duck genetic breeding.
|
|
Received: 31 July 2022
|
|
Corresponding Authors:
*liuxiaolin@nwsuaf.edu.cn
|
|
|
|
[1] 胡志刚, 曹俊婷, 张慧琳, 等. 2021. 中国养鸭的历史起源及演变[J]. 中国家禽 , 43(04): 96-102.
(Hu Z G, Cao J T, Zhang H L, et al. 2021. Historical origin and evolution of duck breeding history in China[J]. China Poultry, 43(04): 96-102. )
[2] 林金杏, 毛赟杰, 米玉玲, 等. 2011. Ghrelin 对鸡等级前卵泡发育的影响[J]. 畜牧兽医学报, 42(9): 1220-1226.
(Lin J X, Mao Y J, Mi Y L, et al. 2011. Effect of ghrelin on development of chicken prehierarchical follicles[J]. Acta Veterinaria et Zootechnica Sinica, 42(9): 1220-1226. )
[3] 余道伦, 左瑞华. 2018. 鹅等级卵泡颗粒细胞层形态观察及卵泡激素受体表达研究[J]. 巢湖学院学报, 20(3): 41-46.
(Yu D L, Zuo R H. 2018. Studies of hierarchy follicles granulosa cells layer morphology and follicle hormone receptors expression in goose[J]. Journal of Chaohu College, 20(3): 41-46. )
[4] Adhikari D, Gorre N, Risal S, et al. 2012. The safe use of a PTEN inhibitor for the activation of dormant mouse primordial follicles and generation of fertilizable eggs[J]. PLOS ONE, 7(6): e39034.
[5] Albamonte M I, Calabró L Y, Albamonte M S, et al. 2020. PTEN and FOXO3 expression in the prenatal and postnatal human ovary[J]. Journal of Assisted Reproduction and Genetics, 37(7): 1613-1622.
[6] Aronal A P, Huda N, Ahmad R. 2012. Amino acid and fatty acid profiles of Peking and Muscovy duck meat[J]. International Journal of Poultry Science, 11(3): 229-236.
[7] Chen C Y, Chen J, He L, et al. 2018. PTEN: Tumor suppressor and metabolic regulator[J]. Frontiers in Endocrinology, 9: 338.
[8] De Felici M, Klinger F G. 2021. PI3K/PTEN/AKT signaling pathways in germ cell development and their involvement in germ cell tumors and ovarian dysfunctions[J]. International Journal of Molecular Sciences, 22(18): 9838.
[9] Dupont J, Scaramuzzi R J. 2016. Insulin signalling and glucose transport in the ovary and ovarian function during the ovarian cycle[J]. The Biochemical Journal, 473(11): 1483-1501.
[10] Etches R J, Petitte J N. 1990. Reptilian and avian follicular hierarchies: Models for the study of ovarian development[J]. The Journal of Experimental Zoology, 4: 112-122. Froment P, Bontoux M, Pisselet C, et al. 2005. PTEN expression in ovine granulosa cells increases during terminal follicular growth[J]. FEBS Letters, 579(11): 2376-2382.
[11] Johnson A L. 2014. The avian ovary and follicle development: Some comparative and practical insights[J]. Turkish Journal of Veterinary and Animal Sciences, 38(6): 660-669.
[12] Johnson A L. 2015. Ovarian follicle selection and granulosa cell differentiation[J]. Poultry Science, 9(4): 781-785.
[13] Johnson A L, Lee J. 2016. Granulosa cell responsiveness to follicle stimulating hormone during early growth of hen ovarian follicles[J]. Poultry Science, 95(1): 108-114.
[14] Kirstein A S, Kehr S, Nebe M, et al. 2021. PTEN regulates adipose progenitor cell growth, differentiation, and replicative aging[J]. The Journal of Biological Chemistry, 297(2): 100968.
[15] Li J, Yen C, Liaw D, et al. 1997. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer[J]. Science, 275(5308): 1943-1947.
[16] Li Y, Wang X Y, Wu T, et al. 2013. PTEN is involved in modulation of vasculogenesis in early chick embryos[J]. Biology Open, 2(6): 587-595.
[17] Liang T, Gao F, Chen J. 2021. Role of PTEN-less in cardiac injury, hypertrophy and regeneration[J]. Cell Regeneration, 10(1): 25.
[18] Lin J, Guan L, Ge L, et al. 2021. Nanopore-based full-length transcriptome sequencing of Muscovy duck (Cairina moschata) ovary[J]. Poultry Science, 100(8): 101246.
[19] Liu T, Wang Y, Wang Y, et al. 2019. Multifaceted regulation of PTEN subcellular distributions and biological functions[J]. Cancers (Basel), 11(9):1247.
[20] Mclanghlin M, Innell H L, Anderson R A, et al. 2014. Inhibition of phosphatase and tensin homologue (PTEN) in human ovary in vitro results in increased activation of primordial follicles but compromises development of growing follicles[J]. Molecular Human Reproduction, 20(8): 736-744.
[21] Naderali E, Khaki A A, Rad J S, et al. 2018. Regulation and modulation of PTEN activity[J]. Molecular Biology Reports, 45(6): 2869-2881.
[22] Noorolyai S, Shajari N, Baghbani E, et al. 2019. The relation between PI3K/AKT signalling pathway and cancer[J]. Gene, 698(2): 120-128.
[23] Ogino M, Ichimura M, Nakano N, et al. 2016. Roles of PTEN with DNA repair in Parkinson's disease[J]. International Journal of Molecular Sciences, 17(6): 954.
[24] Oktay K, Turan V, Titus S, et al. 2015. BRCA mutations, DNA repair deficiency, and ovarian aging[J]. Biology of Reproduction, 93(3): 67.
[25] Onagbesan O, Bruggeman V, Decuypere E. 2009. Intra-ovarian growth factors regulating ovarian function in avian species: A review[J]. Animal Reproduction Science, 111(2-4): 121-140.
[26] Ortega-Molina A, Serrano M. 2013. PTEN in cancer, metabolism, and aging[J]. Trends in Endocrinology and Metabolism, 24(4): 184-189.
[27] Papa A, Pandolfi P P. 2019. The PTEN-PI3K axis in cancer[J]. Biomolecules, 9(4): 153.
[28] Park M K, Yao Y, Xia W, et al. 2019. PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression[J]. Nature Communications, 10(1): 636.
[29] Prvanović M, Nedeljković M, Tanić N, et al. 2021. Role of PTEN, PI3K, and mTOR in triple-negative breast cancer[J]. Life (Basel), 11(11): 1247.
[30] Pulido R. 2018. PTEN inhibition in human disease therapy[J]. Molecules, 23(2): 285.
[31] Rangel P L, Rodríguez A, Gutiérrez K, et al. 2014. Subdominant hierarchical ovarian follicles are needed for steroidogenesis and ovulation in laying hens (Gallus domes- ticus)[J]. Animal Reproduction Science, 147(3-4): 144-153.
[32] Scanga S E, Ruel L, Binari R C, et al. 2000. The conserved PI3K/PTEN/Akt signaling pathway regulates both cell size and survival in Drosophila[J]. Oncogene, 19(35): 3971-3977.
[33] Wang X, Huang H, Young K H. 2015. The PTEN tumor suppressor gene and its role in lymphoma pathogenesis[J]. Aging, 7(12):1032-1049.
[34] Yu D, Zhang L, Wang H, et al. 2019. A potential role for SMAD9 in goose follicular selection through regulation of mRNA levels of luteinizing hormone receptor[J]. The riogenology, 135: 204-212.
[35] Zhang J, Li L, Peng Y, et al. 2018. Surface chemistry induces mitochondria-mediated apoptosis of breast cancer cells via PTEN/PI3K/AKT signaling pathway[J]. Biochimica et Biophysica Acta. Molecular Cell Research, 1865(1): 172-185. |
|
|
|
