Analysis of Effects of Different Concentrate/Forage Ratio Diets on Lipid Metabolism in Femoral and Omental Adipose Tissue of Sheep (Ovis aries) Based on Transcriptome Data
YANG Jin-Li*, WU Meng-Nan, GAO Zhi-Xiong, WANG Ya-Ning, LI Rui-Xue, SHAO Yu-Meng, WANG Hai-Rong, GAO Ai-Wu
Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract Feeding high-concentrate diets often causes excessive visceral fat deposition and high incidence of metabolic diseases in ruminants. In this study, high throughput sequencing technology was used to systematically analyze the lipid metabolism of femoral and omental adipose tissue in sheep fed with different concentrate to forage ratios diets. 15 healthy Dumont male lambs (Ovis aries) aged (90±15) days were selected and randomly divided into 3 groups. Diets concentrate ratio were 30% (G30), 50% (G50), and 70% (G70). After 15 d of adaptation, sheep were raised for 60 d and slaughtered at the end. Femoral and omental fat from 3 lamb that close to average body weight of every group were sampled for transcriptome sequencing. KEGG pathway enrichment analysis were conducted to study more than 10% differentially expressed gene (DEG) between different groups. Relative gene expression of lipid metabolism related key enzymes in femoral fat were also determined by qPCR. The results showed that the dry matter intake of sheep in G50 group were significantly (P<0.05) higher than those in G70 and G30 groups. The body weight of sheep in G50 and G70 groups after 60 days of feeding was significantly (P<0.05) higher than that in G30 group. KEGG pathway enrichment result and essential gene expression changes combined to indicate fatty acid synthesis, elongation, desaturation, triglyceride metabolism and fatty acid degradation in femoral and omental fat of G50 group were all higher than those in the G30 group. The fatty acid elongation, desaturation and fatty acid degradation of femoral and omental fat in G70 group were higher than those in G30 group. The fatty acid synthesis and desaturation of femoral and omental fat in G50 group were higher than those in G70 group. The differential impact of diets concentrate ratio on femoral or omental fat were as follow: The triglyceride metabolism of omental fat in G70 group was higher than that in G30 group (Padjust<0.05). The fatty acid elongation and triglyceride metabolism of femoral fat in G50 group were higher (Padjust<0.05) than those in G70 group. The fatty acid degradation of omental fat in G50 group was significantly (Padjust<0.05) higher than that in G70 group. qPCR results showed that the relative expression of fatty acid synthase and stearoyl CoA desaturase in femoral fat of G50 group were significantly higher than those in G30 and G70 groups (P<0.05), which was consistent with the transcriptome results. This study revealed the similarities and differences in lipid metabolism of femoral fat and omental fat of sheep fed with different concentrate ratio diets which may provide insights for reducing visceral fat deposit and increasing feed efficience.
YANG Jin-Li,WU Meng-Nan,GAO Zhi-Xiong, et al. Analysis of Effects of Different Concentrate/Forage Ratio Diets on Lipid Metabolism in Femoral and Omental Adipose Tissue of Sheep (Ovis aries) Based on Transcriptome Data[J]. 农业生物技术学报, 2023, 31(6): 1206-1217.
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